Review




Structured Review

Valiant Co Ltd core kit
Core Kit, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/taq+core+kit+25/Taq+CORE+kit+25/pm39082812-279-42-45
Average 96 stars, based on 9 article reviews
core kit - by Bioz Stars, 2026-09
96/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Characterization of a cassiicolin-encoding gene from Corynespora cassiicola, pathogen of rubber tree (Hevea brasiliensis).
Article Snippet: Corynespora Leaf Fall (CLF) is a major disease of rubber tree (Hevea brasiliensis) caused by the Ascomycota Corynespora cassiicola.. Here we describe the cloning and characterization of a gene encoding cassiicolin (Cas), a glycosylated cystein-rich small secreted protein (SSP) identified as a potential CLF disease effector in rubber tree.. Three isolates with contrasted levels of aggressiveness were analyzed comparatively.

Article Title: First molecular detection of Mycobacterium bovis in environmental samples from a French region with endemic bovine tuberculosis.
Article Snippet: .. Positive DNA extracts were then tested by the nested IS15610 PCR protocol as follows: the first PCR was achieved in a 25 ll reaction mixture containing 17 25 ll of ultrapure water for molecular biology, 2 5 ll of PCR Buffer 109 with MgCl2, 0 2 ll of dNTP mix at 25 mmol l 1 each, 0 05 ll of Taq DNA polymerase 15 U ll 1 (Taq Core Kit 25; MP Biomedicals Europe), 1 25 ll of each primers (final concentration 500 nmol l 1) and 2 5 ll of environmental DNA. .. The nested PCR was achieved in a Veriti Thermal Cycler (Applied Biosystems, -Thermo Fisher Scientific, Courtaboeuf, France) with a first step of denaturation at 94°C for 5 min, then 30 cycles for 30 s at 94°C for denaturation, 30 s at 60°C for annealing and 30 s at 72°C for elongation, and 5 min at 72°C for final elongation.

Reverse Transcription:

Article Title: Characterization of a cassiicolin-encoding gene from Corynespora cassiicola, pathogen of rubber tree (Hevea brasiliensis).
Article Snippet: Corynespora Leaf Fall (CLF) is a major disease of rubber tree (Hevea brasiliensis) caused by the Ascomycota Corynespora cassiicola.. Here we describe the cloning and characterization of a gene encoding cassiicolin (Cas), a glycosylated cystein-rich small secreted protein (SSP) identified as a potential CLF disease effector in rubber tree.. Three isolates with contrasted levels of aggressiveness were analyzed comparatively.

Amplification:

Article Title: Characterization of a cassiicolin-encoding gene from Corynespora cassiicola, pathogen of rubber tree (Hevea brasiliensis).
Article Snippet: Corynespora Leaf Fall (CLF) is a major disease of rubber tree (Hevea brasiliensis) caused by the Ascomycota Corynespora cassiicola.. Here we describe the cloning and characterization of a gene encoding cassiicolin (Cas), a glycosylated cystein-rich small secreted protein (SSP) identified as a potential CLF disease effector in rubber tree.. Three isolates with contrasted levels of aggressiveness were analyzed comparatively.

Nested PCR:

Article Title: Optimizing TILLING populations for reverse genetics in Medicago truncatula.
Article Snippet: .. The nested PCR was performed in 11-μL volumes (5 μL of DNA + 6 μL of mix) using the Taq Core kit 25 [including concentrated Taq (15 U/μL), deoxynucleoside triphosphate (dNTP) and 10 × buffer] from MP-QBiogene (MP Biomedicals, France). ..

Concentration Assay:

Article Title: First molecular detection of Mycobacterium bovis in environmental samples from a French region with endemic bovine tuberculosis.
Article Snippet: .. Positive DNA extracts were then tested by the nested IS15610 PCR protocol as follows: the first PCR was achieved in a 25 ll reaction mixture containing 17 25 ll of ultrapure water for molecular biology, 2 5 ll of PCR Buffer 109 with MgCl2, 0 2 ll of dNTP mix at 25 mmol l 1 each, 0 05 ll of Taq DNA polymerase 15 U ll 1 (Taq Core Kit 25; MP Biomedicals Europe), 1 25 ll of each primers (final concentration 500 nmol l 1) and 2 5 ll of environmental DNA. .. The nested PCR was achieved in a Veriti Thermal Cycler (Applied Biosystems, -Thermo Fisher Scientific, Courtaboeuf, France) with a first step of denaturation at 94°C for 5 min, then 30 cycles for 30 s at 94°C for denaturation, 30 s at 60°C for annealing and 30 s at 72°C for elongation, and 5 min at 72°C for final elongation.



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